GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 

Pathway Map

CHST3



General Information
Gene Symbol:   
CHST3
Protein name:   
Carbohydrate sulfotransferase 3
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   
PAPS +
+ PAP
PAPS +
+ PAP
Reaction (IUBMB):   
No specified reaction.

About

• Chondroitin sulfate chains are composed of GlcAβ1-3GalNAcβ1-4 repeating unit. Various sulfotransferases, CHST3, 7, 11, 12, 13, 14, 15, and UST, sulfate either GalNAc and GlcA residues. CS are a major functional component of aggrecan, and needed for cartilage formation and maintenance. It is also involved in interactions with growth factors pleiotrophin and midkine.
• CHST3 (C6ST-1) and CHST7 (C6ST-2) are Chondroitin 6-sulfotransferases that attach sulfate to C6 of GalNAc. They are both type II transmembrane proteins.
• CHST3 can also sulfate C6 of Gal in keratin sulfate and sialyl LacNAc oligonucleotides, but not SLeX. It may also sulfate GAG protein linkage. This is not the only sulfoT capable of modifying keratin sulfate.
• C4-sulfation prevents C6-sulfation by CHST3.
• CHST3 KO-mice are fertile and show no obvious pathological features. Some repair mechanisms were impaired.
• CHST3 and CHST15 are secreted into culture medium.


Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
Reactome:   

Related Database
Organism:   
Homo sapiens
Uniprot:   
NCBI GeneID:   
GlyMap GeneID:   
DNA aRefSeq:   
Protein RefSeq:   
BRENDA:   
OMIM number:   
GeneCards database:   

Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcA(b1-3)GalNAc(b1-4)GlcA(b1-3) or NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-3 [6S])
Product:  
     GlcA(b1-3)GalNAc(b1-4 [6S])GlcA(b1-3) or NeuAc(a2-3)Gal(b1-4 [6S])GlcNAc(b1-3 [6S])
Constraints:  
     var=50%

Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN











Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000373115 TFAP2C REST NFIC SP1 ZFX MYC ZBTB26 ZNF600 GATA2 MAX MAZ ZBTB33 ZSCAN9 ZNF335 ZFP64 TEAD4 REST EGR3 EGR2 EGR2 MAZ SP3 KLF15 WT1 SP5 ZNF398 ZNF263 SP4 ZNF467 KLF9


miRNA-GlycoEnzyme Interactome {ref. 9}
CHST3/hsa-miR-15a-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...GAACCCAACUCCUG AUGGCCUA ...
3'   ACUCCGUCGUGUUA UACCGGA C
8mer 5037-5044 -0.326 98.0 -0.316 99.0 noncon