GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST14



General Information
Gene Symbol:   
CHST14
Protein name:   
Carbohydrate sulfotransferase 14
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.35
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Chondroitin sulfate chains are composed of GlcAβ1-3GalNAcβ1-4 repeating unit. Various sulfotransferases, CHST3, 7, 11, 12, 13, 14, 15, and UST, sulfate either GalNAc and GlcA residues. CS are a major functional component of aggrecan, and needed for cartilage formation and maintenance.
• Dermatan 4-O sulfotransferase 1 (D4ST1) is the CS/DS 4-O-sulfotransferase responsible for the sulfation of GalNAc in blocks of IdoA-GalNAc-IdoA.
• It acts both nearly fully or partially desulfated DS, but not on CS.
• Chst14/D4st1-deficient mice showed growth retardation as well as multiple system abnormalities including neurological changes.

Related Pathway
KEGG ORTHOLOGY:   
K08105
KEGG Pathway:   
ko00532 
Reactome:   
R-HSA-2022923
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8NCH0 
NCBI GeneID:   
113189
GlyMap GeneID:   
113189
DNA aRefSeq:   
NM_130468.3 
Protein RefSeq:   
NP_569735.1 
BRENDA:   
Kinetic info.
OMIM number:   
608429
GeneCards database:   
CHST14
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-3)GalNAc(b1-4)IdoA(a1-3) or IdoA(a1-3 [2S])GalNAc(b1-4)IdoA(a1-3) or IdoA(a1-3)GalNAc(b1-4)IdoA(a1-3 [2S]) or IdoA(a1-3 [2S])GalNAc(b1-4)IdoA(a1-3 [2S])
Product:  
     IdoA(a1-3)GalNAc(b1-4 [4S])IdoA(a1-3) or IdoA(a1-3 [2S])GalNAc(b1-4 [4S])IdoA(a1-3) or IdoA(a1-3)GalNAc(b1-4 [4S])IdoA(a1-3 [2S]) or IdoA(a1-3 [2S])GalNAc(b1-4 [4S])IdoA(a1-3 [2S])
Constraints:  
     var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
No expression data available.
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
CHST14/hsa-miR-5581-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...UUCUGGCACUACAA GGAAGGCA ...
3'   AGAGGUAAAGAGGA CCUUCCG A
 8mer 1549-1556 -0.346 95.0 -0.002 28.0 noncon