GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST10



General Information
Gene Symbol:   
CHST10
Protein name:   
Carbohydrate sulfotransferase 10
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Terminal 3-O-sulfation of GlcA on the HNK-1 epitope. It may also sulfate GlcA to terminate extension of GAGs and Dystroglycans. after one of the glucuronyltransferases.
• CHST10 would act on the gene product of B3GAT1 and/or B3GAT2 that are involved in the biosynthesis of the HNK-1 epitope.
• HNK-1 carbohydrate structure is found on cell adhesion molecules like NCAM, MAG, L1, and P0.
• No human diseases have been associated with the HNK-1 sulfotransferase, but mice lacking CHST10 exhibit learning and memory deficiency.

Related Pathway
KEGG ORTHOLOGY:   
K09674
KEGG Pathway:   
ko00515  ko01100 
Reactome:   
R-HSA-975578
Related Database
Organism:   
Homo sapiens
Uniprot:   
O43529 
NCBI GeneID:   
9486
GlyMap GeneID:   
9486
DNA aRefSeq:   
NM_004854.4 
Protein RefSeq:   
NP_004845.1 
BRENDA:   
Kinetic info.
OMIM number:   
606376
GeneCards database:   
CHST10
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcA(b1-3)Gal(b1-4)GlcNAc(b1-?)
Product:  
     GlcA(b1-3 [3S]])Gal(b1-4)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-96-5p 1373-1380 -0.87 99.0 -0.104 83.0 con
hsa-miR-1271-5p 1373-1380 -0.867 99.0 -0.104 82.0 con
hsa-miR-3195 848-855 -0.65 96.0 -0.65 97.0 noncon
hsa-miR-138-5p 1088-1095 -0.566 99.0 -0.566 99.0 con
hsa-miR-6825-3p 589-596 -0.465 98.0 -0.465 98.0 noncon
hsa-miR-183-5p.2 1373-1379 -0.461 98.0 -0.062 71.0 con
hsa-miR-483-3p.1 495-502 -0.442 99.0 -0.442 99.0 noncon
hsa-miR-6836-3p 545-552 -0.422 98.0 -0.422 99.0 noncon
hsa-miR-3127-3p 760-767 -0.39 97.0 -0.39 98.0 noncon
hsa-miR-4452 15-22 -0.385 99.0 -0.385 99.0 noncon
hsa-miR-6880-5p 82-89 -0.373 97.0 -0.373 97.0 noncon
hsa-miR-6868-5p 1021-1028 -0.372 98.0 -0.372 98.0 noncon
hsa-miR-4437 61-68 -0.363 97.0 -0.363 97.0 noncon
hsa-miR-6756-3p 760-767 -0.358 96.0 -0.358 97.0 noncon
hsa-miR-378g 63-70 -0.355 98.0 -0.355 98.0 noncon
hsa-miR-3127-3p 347-354 -0.348 96.0 -0.348 97.0 noncon
hsa-miR-6887-3p 736-743 -0.346 94.0 -0.346 95.0 noncon
hsa-miR-184 1191-1197 -0.343 90.0 -0.343 92.0 noncon
hsa-miR-6756-3p 347-354 -0.34 96.0 -0.34 97.0 noncon
hsa-miR-4741 287-294 -0.337 98.0 -0.337 98.0 noncon
hsa-miR-4323 39-46 -0.329 97.0 -0.329 98.0 noncon
hsa-miR-6765-3p 48-55 -0.324 98.0 -0.324 99.0 noncon
hsa-miR-3943 453-459 -0.321 96.0 -0.321 97.0 noncon
hsa-miR-6749-3p 298-305 -0.319 95.0 -0.319 96.0 noncon
hsa-miR-210-5p 944-951 -0.315 96.0 -0.315 97.0 noncon
hsa-miR-3170 156-163 -0.311 96.0 -0.311 97.0 noncon
hsa-miR-23b-3p 1348-1355 -0.309 95.0 -0.309 96.0 con
hsa-miR-23c 1348-1355 -0.309 95.0 -0.309 96.0 con
hsa-miR-23a-3p 1348-1355 -0.309 95.0 -0.309 96.0 con
hsa-miR-130a-5p 1348-1355 -0.299 95.0 -0.299 96.0 con
hsa-miR-4493 516-523 -0.298 95.0 -0.298 96.0 noncon
hsa-miR-760 1025-1032 -0.291 93.0 -0.291 95.0 noncon
hsa-miR-6855-5p 156-163 -0.29 95.0 -0.29 96.0 noncon
hsa-miR-4251 1321-1328 -0.287 98.0 -0.287 98.0 noncon
hsa-miR-409-5p 1076-1083 -0.287 92.0 -0.287 95.0 noncon
hsa-miR-412-3p 51-58 -0.287 96.0 -0.287 97.0 noncon
hsa-miR-6088 1001-1008 -0.284 97.0 -0.284 98.0 con
hsa-miR-4529-5p 595-602 -0.284 92.0 -0.284 94.0 noncon
hsa-miR-6501-3p 191-198 -0.283 97.0 -0.283 98.0 noncon
hsa-miR-744-3p 1139-1146 -0.281 95.0 -0.281 96.0 noncon
hsa-miR-7974 1196-1203 -0.274 92.0 -0.274 94.0 noncon
hsa-miR-182-5p 1374-1380 -0.274 93.0 -0.039 56.0 con
hsa-miR-137 1092-1099 -0.273 91.0 -0.273 94.0 con
hsa-miR-7851-3p 1109-1116 -0.272 93.0 -0.272 96.0 noncon
hsa-miR-4675 287-294 -0.272 98.0 -0.272 98.0 noncon
hsa-miR-6754-3p 51-58 -0.269 96.0 -0.269 97.0 noncon
hsa-miR-6721-5p 203-210 -0.264 92.0 -0.264 93.0 noncon
hsa-miR-371b-3p 1016-1022 -0.263 92.0 -0.263 95.0 noncon
hsa-miR-383-5p.1 1035-1042 -0.263 92.0 -0.263 94.0 noncon
hsa-miR-514a-3p 1343-1349 -0.26 96.0 -0.26 97.0 noncon
hsa-miR-514b-3p 1343-1349 -0.26 96.0 -0.26 97.0 noncon
hsa-miR-5006-5p 1136-1143 -0.255 93.0 -0.255 95.0 noncon
hsa-miR-6840-3p 1228-1235 -0.254 98.0 -0.254 99.0 noncon
hsa-miR-6778-3p 740-746 -0.254 94.0 -0.254 96.0 noncon
hsa-miR-3173-5p 1160-1167 -0.252 93.0 -0.252 95.0 noncon
hsa-miR-7847-3p 81-87 -0.251 90.0 -0.251 92.0 noncon
hsa-miR-1178-5p 73-79 -0.25 91.0 -0.25 94.0 noncon
hsa-miR-194-5p 164-171 -0.25 95.0 -0.25 97.0 noncon
hsa-miR-143-3p 1001-1008 -0.25 96.0 -0.25 97.0 con