GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST1



General Information
Gene Symbol:   
CHST1
Protein name:   
Carbohydrate sulfotransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
E.C. No:   
2.8.2.21
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Keratan sulfate (KS) is composed of repeating disaccharide LacNAc units (Galβ1-4GlcNAc) with C6 sulfation of both Gal and GlcNAc. GlcNAc sulfation often exceeds Gal sulfation suggesting that GlcNAc sulfation may occur first.
• CHST1 has C6 Gal sulfation properties (both for unsulfated LacNAc substrate and also sulfated KS).
• In addition to KS, this enzyme may also contribute to the formation of glycoproteins bearing C6-Gal sulfated sialyl N-acetyllactosamine or sulfated Sialyl-Lewis X oligosaccharides. These glycan structures can act as selectin ligands during lymphocyte homing to HEV (high endothelial venules).
• The enzyme does not act on CS, DS or HS.
• In vitro synthesis of KS has been achieved by mixing some of these sulfotransferases with B4GalT1,4, along with B3GNT2 and 7 (for lactosamine chain extension).

Related Pathway
KEGG ORTHOLOGY:   
K01022
KEGG Pathway:   
ko00533 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
O43916 
NCBI GeneID:   
8534
GlyMap GeneID:   
8534
DNA aRefSeq:   
NM_003654.5 
Protein RefSeq:   
NP_003645.1 
BRENDA:   
Kinetic info.
OMIM number:   
603797
GeneCards database:   
CHST1
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-3)Gal(b1-4)GlcNAc(b1-3 [6S]) or NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-? [6S]) or NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-?)
Product:  
     GlcNAc(b1-3)Gal(b1-4 [6S])GlcNAc(b1-3 [6S]) or NeuAc(a2-3)Gal(b1-4 [6S])GlcNAc(b1-? [6S]) or NeuAc(a2-3)Gal(b1-4 [6S])GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1247-5p 180-187 -0.716 99.0 -0.716 99.0 noncon
hsa-miR-25-3p 801-808 -0.632 99.0 -0.632 99.0 con
hsa-miR-1271-5p 712-719 -0.627 99.0 -0.627 99.0 con
hsa-miR-363-3p 801-808 -0.621 99.0 -0.621 99.0 con
hsa-miR-367-3p 801-808 -0.621 99.0 -0.621 99.0 con
hsa-miR-32-5p 801-808 -0.611 99.0 -0.611 99.0 con
hsa-miR-7703 799-806 -0.61 99.0 -0.61 99.0 noncon
hsa-miR-96-5p 712-719 -0.606 99.0 -0.606 99.0 con
hsa-miR-7108-3p 19-26 -0.603 99.0 -0.603 99.0 noncon
hsa-miR-92a-3p 801-808 -0.595 99.0 -0.595 99.0 con
hsa-miR-92b-3p 801-808 -0.595 99.0 -0.595 99.0 con
hsa-miR-668-5p 25-32 -0.579 98.0 -0.579 98.0 noncon
hsa-miR-639 508-515 -0.557 97.0 -0.557 98.0 noncon
hsa-miR-6777-3p 731-738 -0.554 99.0 -0.554 99.0 noncon
hsa-miR-4295 166-173 -0.508 99.0 -0.508 99.0 con
hsa-miR-3666 166-173 -0.5 99.0 -0.5 99.0 con
hsa-miR-301a-3p 166-173 -0.484 99.0 -0.484 99.0 con
hsa-miR-301b-3p 166-173 -0.484 99.0 -0.484 99.0 con
hsa-miR-130b-3p 166-173 -0.484 99.0 -0.484 99.0 con
hsa-miR-130a-3p 166-173 -0.476 99.0 -0.476 99.0 con
hsa-miR-454-3p 166-173 -0.455 99.0 -0.455 99.0 con
hsa-miR-1257 1979-1986 -0.435 99.0 -0.001 25.0 noncon
hsa-miR-4764-5p 302-309 -0.42 98.0 -0.42 98.0 noncon
hsa-miR-3691-5p 304-311 -0.415 97.0 -0.415 98.0 noncon
hsa-miR-152-3p 382-388 -0.408 98.0 -0.408 98.0 con
hsa-miR-148b-3p 382-388 -0.408 98.0 -0.408 98.0 con
hsa-miR-148a-3p 382-388 -0.408 98.0 -0.408 98.0 con
hsa-miR-1260b 910-917 -0.406 98.0 -0.001 28.0 noncon
hsa-miR-1260a 910-917 -0.406 98.0 -0.001 28.0 noncon
hsa-miR-4662b 1941-1948 -0.405 99.0 -0.001 37.0 noncon
hsa-miR-183-5p.2 712-718 -0.403 97.0 -0.403 98.0 con
hsa-miR-6887-5p 496-502 -0.396 91.0 -0.396 94.0 noncon
hsa-miR-124-3p.2 71-77 -0.381 95.0 -0.381 97.0 con
hsa-miR-6134 94-101 -0.375 97.0 -0.375 98.0 noncon
hsa-miR-6795-5p 496-502 -0.374 90.0 -0.374 93.0 noncon
hsa-miR-4688 143-149 -0.366 97.0 -0.366 98.0 noncon
hsa-miR-324-3p 194-201 -0.365 97.0 -0.365 98.0 noncon
hsa-miR-553 56-62 -0.365 96.0 -0.365 97.0 noncon
hsa-miR-486-3p 142-148 -0.363 93.0 -0.363 95.0 noncon
hsa-miR-6853-3p 750-757 -0.361 98.0 -0.361 98.0 noncon
hsa-miR-6743-5p 143-149 -0.356 97.0 -0.356 98.0 noncon
hsa-miR-891a-3p 1991-1997 -0.356 97.0 -0.001 31.0 noncon
hsa-miR-3141 531-537 -0.355 95.0 -0.355 96.0 noncon
hsa-miR-3911 306-313 -0.352 97.0 -0.352 98.0 noncon
hsa-miR-6806-3p 670-676 -0.346 98.0 -0.346 98.0 noncon
hsa-miR-6731-5p 89-96 -0.345 97.0 -0.345 97.0 noncon
hsa-miR-3666 381-387 -0.343 98.0 -0.343 98.0 con
hsa-miR-6804-5p 951-958 -0.34 94.0 -0.001 16.0 noncon
hsa-miR-1913 194-201 -0.339 97.0 -0.339 98.0 noncon
hsa-miR-4422 769-776 -0.335 99.0 -0.335 99.0 noncon
hsa-miR-19a-3p 165-171 -0.334 96.0 -0.334 97.0 con
hsa-miR-19b-3p 165-171 -0.334 96.0 -0.334 97.0 con
hsa-miR-3151-3p 736-742 -0.332 96.0 -0.332 97.0 noncon
hsa-miR-8085 89-96 -0.329 96.0 -0.329 97.0 noncon
hsa-miR-4728-5p 83-89 -0.325 97.0 -0.325 97.0 noncon
hsa-miR-3928-5p 670-676 -0.324 97.0 -0.324 98.0 noncon
hsa-miR-1224-3p 909-916 -0.322 97.0 -0.001 33.0 noncon
hsa-miR-124-3p.2 1853-1860 -0.322 92.0 -0.001 13.0 noncon
hsa-miR-5194 81-87 -0.322 97.0 -0.322 98.0 noncon
hsa-miR-4426 1941-1948 -0.319 99.0 -0.001 50.0 noncon
hsa-miR-6805-5p 519-525 -0.313 91.0 -0.313 93.0 noncon
hsa-miR-3651 1800-1806 -0.312 94.0 -0.001 22.0 noncon
hsa-miR-6778-5p 91-97 -0.31 94.0 -0.31 95.0 noncon
hsa-miR-6738-5p 81-87 -0.308 96.0 -0.308 97.0 noncon
hsa-miR-506-3p 71-77 -0.308 95.0 -0.308 96.0 con
hsa-miR-4790-3p 1964-1971 -0.307 97.0 -0.001 26.0 noncon
hsa-miR-1237-5p 118-124 -0.304 93.0 -0.304 94.0 noncon
hsa-miR-182-5p 713-719 -0.303 95.0 -0.303 97.0 con
hsa-miR-585-5p 1422-1429 -0.302 94.0 -0.001 13.0 noncon
hsa-miR-454-3p 381-387 -0.302 97.0 -0.302 97.0 con
hsa-miR-1207-5p 469-475 -0.3 95.0 -0.3 96.0 noncon
hsa-miR-4487 122-129 -0.297 95.0 -0.297 96.0 noncon
hsa-miR-4697-5p 118-124 -0.296 92.0 -0.296 93.0 noncon
hsa-miR-149-3p 83-89 -0.293 95.0 -0.293 96.0 noncon
hsa-miR-6785-5p 83-89 -0.292 96.0 -0.292 96.0 noncon
hsa-miR-3910 1852-1859 -0.292 98.0 -0.001 30.0 noncon
hsa-miR-5579-5p 1962-1968 -0.292 96.0 -0.001 29.0 noncon
hsa-miR-1324 618-625 -0.292 98.0 -0.292 98.0 noncon
hsa-miR-19a-3p 380-386 -0.291 94.0 -0.291 95.0 con
hsa-miR-4258 20-26 -0.29 92.0 -0.29 94.0 noncon
hsa-miR-30c-5p 281-288 -0.29 94.0 -0.29 96.0 con
hsa-miR-30b-5p 281-288 -0.29 94.0 -0.29 96.0 con
hsa-miR-130b-3p 381-387 -0.288 96.0 -0.288 97.0 con
hsa-miR-1233-5p 91-97 -0.288 93.0 -0.288 94.0 noncon
hsa-miR-130a-3p 381-387 -0.288 96.0 -0.288 97.0 con
hsa-miR-196a-3p 68-74 -0.285 94.0 -0.285 96.0 noncon
hsa-miR-4488 118-124 -0.284 91.0 -0.284 93.0 noncon
hsa-miR-6784-3p 356-362 -0.282 94.0 -0.282 96.0 noncon
hsa-miR-6883-5p 83-89 -0.282 95.0 -0.282 96.0 noncon
hsa-miR-6812-5p 128-134 -0.281 92.0 -0.281 94.0 noncon
hsa-miR-19b-3p 380-386 -0.28 93.0 -0.28 95.0 con
hsa-miR-660-3p 1818-1825 -0.279 97.0 -0.001 26.0 noncon
hsa-miR-4647 1941-1948 -0.279 99.0 -0.001 51.0 noncon
hsa-miR-203b-5p 424-430 -0.279 95.0 -0.279 96.0 noncon
hsa-miR-301a-3p 381-387 -0.277 95.0 -0.277 96.0 con
hsa-miR-301b-3p 381-387 -0.277 95.0 -0.277 96.0 con
hsa-miR-30e-5p 281-288 -0.274 93.0 -0.274 95.0 con
hsa-miR-30a-5p 281-288 -0.274 94.0 -0.274 95.0 con
hsa-miR-30d-5p 281-288 -0.274 94.0 -0.274 95.0 con
hsa-miR-6129 85-91 -0.273 93.0 -0.273 95.0 noncon
hsa-miR-6133 85-91 -0.273 93.0 -0.273 95.0 noncon
hsa-miR-6718-5p 424-430 -0.268 95.0 -0.268 96.0 noncon
hsa-miR-7152-3p 643-649 -0.267 93.0 -0.267 95.0 noncon
hsa-miR-642a-3p 1812-1819 -0.266 96.0 -0.001 26.0 noncon
hsa-miR-642b-3p 1812-1819 -0.266 96.0 -0.001 26.0 noncon
hsa-miR-4295 381-387 -0.266 95.0 -0.266 96.0 con
hsa-miR-6127 85-91 -0.262 92.0 -0.262 94.0 noncon
hsa-miR-506-3p 1853-1860 -0.262 91.0 -0.001 18.0 noncon
hsa-miR-1255b-2-3p 1359-1366 -0.26 96.0 -0.001 28.0 noncon
hsa-miR-636 287-293 -0.258 95.0 -0.258 96.0 noncon
hsa-miR-4763-3p 469-475 -0.257 93.0 -0.257 94.0 noncon
hsa-miR-6862-3p 356-362 -0.257 93.0 -0.257 95.0 noncon
hsa-miR-6130 85-91 -0.251 91.0 -0.251 93.0 noncon
hsa-miR-4510 85-91 -0.251 91.0 -0.251 93.0 noncon