GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
C1GALT1



General Information
Gene Symbol:   
C1GALT1
Protein name:   
Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.122
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Forms T-antigen. In the absence of this, we are left with the Tn-antigen.
• Core-2 O-glycans are not formed in the absence of this enzyme, and in leukocytes this results in reduced cell adhesion.
• Absence of T-antigen promotes metastasis in a number of models including pancreatic cancer.
• Tn-syndrome is a rare hematological disorder characterized by the expression of the Tn-antigen on a subpopulation of blood cells in all lineages.
• Active T-synthase in vertebrates, including humans, requires a specific molecular chaperone Cosmc (Core-1 β3galactosyltransferase Specific Molecular Chaperone) or C1GALT1C1 (Core-1 β3Galactosyltransferase Chaperone-1) for its folding in vivo.
• T-synthase is localized in Golgi apparatus, Cosmc mainly resides in the endoplasmic reticulum (ER).
• Knocking out C1GalT1 activity in mice results in severe hematological disorders.

Related Pathway
KEGG ORTHOLOGY:   
K00731
KEGG Pathway:   
ko00512  ko01100 
Reactome:   
R-HSA-5083632
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9NS00 
NCBI GeneID:   
56913
GlyMap GeneID:   
56913
DNA aRefSeq:   
NM_020156.4 
Protein RefSeq:   
NP_064541.1 
BRENDA:   
Kinetic info.
OMIM number:   
610555
GeneCards database:   
C1GALT1
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type)
Compartment:  
     
Substrate:  
     GalNAc(a1-?)Ser|Thr
Product:  
     Gal(b1-3)GalNAc(a1-?)Ser|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
C1GALT1/hsa-miR-29a-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AACAGUCUGAAGGU UGGUGCUA ...
3'   AUUGGCUAAAGUCU ACCACGA U
 8mer 1186-1193 -0.271 90.0 -0.06 66.0 noncon