GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B4GALT5



General Information
Gene Symbol:   
B4GALT5
Protein name:   
Beta-1,4-galactosyltransferase 5
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT7
E.C. No:   
2.4.1.90
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Weak enzyme activity. β4GalT5 mainly produces mono-galactosylated product. Prefers to transfer Gal to β1,4GlcNAc arm at α1,3-mannose.

Related Pathway
KEGG ORTHOLOGY:   
K09905
KEGG Pathway:   
ko00512 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
O43286 
NCBI GeneID:   
9334
GlyMap GeneID:   
9334
DNA aRefSeq:   
NM_004776.3 
Protein RefSeq:   
NP_004767.1 
BRENDA:   
Kinetic info.
OMIM number:   
604016
GeneCards database:   
B4GALT5
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-?) or Glc(b1-?)Cer
Product:  
     Gal(b1-4)GlcNAc(b1-?) or Gal(b1-4)Glc(b1-?)Cer
Constraints:  
     !@GlcNAc(b1-6)[GlcNAc(b1-3)]GalNAc(a1-r)&!Gal(b1-3)GlcNAc(b1-?)&!@GlcNAc(b1-4)...Man(b1-4)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    5.75e-04    (pmol/cell • hr)       KM:    1.50e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000371711 FOXP1 ZNF623 JUNB AR GATA1 CTCF KMT2A RUNX1 KLF5 ASCL1 CDX2 ZNF335 DLX1 ZNF816 USF1 MYCN ZNF24 FOSL1 FOSL1 MXI1 MXI1 NCOA2 NCOA2 NCOA2 ZBTB17 ZBTB17 FOS ESRRA ZBTB11 ZNF629 KLF17 L3MBTL4 ZNF580 BACH2 SSRP1 KLF16 SMAD4 E4F1 E4F1 TCF3 GATA6 GATA6 FLI1 NKX2-1 MAFK MAFK NFATC3 ZNF574 SMARCC1 POU2F2 ZEB1 HOXC9 ZKSCAN8 PRDM10 PRDM10 IRF4 MAX MAX ZSCAN22 BATF MXD4 HOMEZ HOMEZ HOMEZ ZBTB26 ARID3A ARID3A KLF7 SP4 ATF7 ATF7 ATF7 TEAD1 DMAP1 ZNF644 ZNF644 GLIS1 ARID4B ARID4B KLF10 KLF10 TFE3 SOX5 TEAD3 RCOR1 SOX13 SOX13 ZNF143 HSF1 GATAD1 IRF3 ZNF48 KLF16 KAT8 ZBTB33 ZBTB33 ZBTB33 SMAD4 SMAD4 ELF1 KLF12 FOSL1 FOSL1 EGR2 HBP1 CEBPG NFIL3 NR2F6 NR2F6 PAX5 CREM CREM SOX9 NFE2 KLF9 MAZ E2F6 E2F6 SKI EGR3 FOXP2 ZBTB14 SMAD1 USF2 EBF1 E2F4 SP2 BHLHE40 GATA1 GATA1 NFE2 CEBPD USF1 KLF9 EGR1 EGR1 RUNX1 TFAP4 VEZF1 CTCFL TAF1 TCF7L1 SP1 E2F6
miRNA-GlycoEnzyme Interactome {ref. 9}
B4GALT5/hsa-miR-7846-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...UGCUCAUCCCACU CUCCGCUA ...
3'   GGAAGAGAGGUCC GAGGCGA C
 8mer 940-947 -0.438 96.0 -0.435 97.0 noncon