GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GNT7



General Information
Gene Symbol:   
B3GNT7
Protein name:   
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• This is a Type II Golgi enzyme that aids the biosynthesis of Keratin sulfates (KS).
• KS is a major component of articular cartilage and cornea, while various KS proteins are also found in other tissues and cells.
• B3GNT7 preferentially acts to on sulfated type 2 glycans (Galβ1-4(SO3-6)GlcNAcβ1-3Galβ1-4(SO3-6)GlcNAc).
• It works in concert with CHST6 and B4GalT4 to extend KS polyLacNAc chains, and its function is reduced upon Gal-sulfation by CHST1.
• B3GNT7 is upregulated during ovarian cancer. It results in the expression of the HMOCC-1 mAb epitope.

Related Pathway
KEGG ORTHOLOGY:   
K09664
KEGG Pathway:   
ko00533 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8NFL0 
NCBI GeneID:   
93010
GlyMap GeneID:   
93010
DNA aRefSeq:   
NM_145236.2 
Protein RefSeq:   
NP_660279.1 
BRENDA:   
Kinetic info.
OMIM number:   
615313
GeneCards database:   
B3GNT7
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)...GlcNAc(b1-?)
Product:  
     GlcNAc(b1-3)Gal(b1-4)...GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.70e-06    (pmol/cell • hr)       KM:    7.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
B3GNT7/hsa-miR-6867-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AAGACAGGAGAAA AGGGAGAA ...
3'   GAUCACCCAUUUC UCCCUCU C
 8mer 1873-1880 -0.376 98.0 -0.376 98.0 noncon
5'...AUGAAGAAUUUGG AGGGAGAA ...
3'   GAUCACCCAUUUC UCCCUCU C
 8mer 876-883 -0.074 66.0 -0.074 77.0 noncon