GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GNT3



General Information
Gene Symbol:   
B3GNT3
Protein name:   
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.149
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• This is the core 1 extension β1,3GnT enzyme. A Golgi localized Type-2 GlycoT.
• Core-1 extension is possible even after core-2 branching.
• The enzyme exhibits no activity for Type 2 and Type 1 LacNAc chains, thus it is O-glycan specific.
• 6-sulfo extended core-1 structure formed by this enzyme along with the sufotransferase GlcNAc6ST-2/CHST4. This extended epitope is called the 'MECA-79 epitope' which is commonly expressed on HEV and secondary lymphoid organs.
• MECA-79 binds lymphocyte L-selectin, and is thus critical for lymphocyte homing.
• Additionally, the enzyme can support the formation of 6'sulfo sLeX type glycans on the core-1 chain.
• Other organs expressing this enzyme include the mucin producing tissue: small and large intestines, stomach, esophagus, trachea and bladder.

Related Pathway
KEGG ORTHOLOGY:   
K07970
KEGG Pathway:   
ko00601  ko01100 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y2A9 
NCBI GeneID:   
10331
GlyMap GeneID:   
10331
DNA aRefSeq:   
NM_014256.3 
Protein RefSeq:   
NP_055071.2 
BRENDA:   
Kinetic info.
OMIM number:   
605863
GeneCards database:   
B3GNT3
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-3)...GalNAc(a1-?)Ser|Thr or Gal(b1-?)GlcNAc(b1-?)
Product:  
     GlcNAc(b1-3)Gal(b1-3)...GalNAc(a1-?)Ser|Thr or GlcNAc(b1-3)Gal(b1-?)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.70e-06    (pmol/cell • hr)       KM:    7.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
B3GNT3/hsa-miR-28-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...CAGAUACCCUUCAU AGCUCCU U...
3'   GAGUUAUCUGACAC UCGAGGA A
 7mer-m8 1421-1427 -0.317 97.0 -0.001 21.0 noncon