GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
A4GNT
ABO
B3GALNT2
B3GALT1
B3GALT2
B3GALT5
B3GAT1
B3GAT2
B3GLCT
B3GNT2
B3GNT3
B3GNT4
B3GNT6
B3GNT7
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
C1GALT1
C1GALT1C1
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
EOGT
FKRP
FKTN
FPGT
FUCA1
FUT1
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT9
GAL3ST2
GAL3ST3
GAL3ST4
GALNT1
GALNT10
GALNT11
GALNT12
GALNT13
GALNT14
GALNT15
GALNT16
GALNT17
GALNT18
GALNT2
GALNT3
GALNT4
GALNT5
GALNT6
GALNT7
GALNT8
GALNT9
GALNTL6
GCNT1
GCNT2
GCNT3
GCNT4
GXYLT1
GXYLT2
LFNG
MFNG
MGAT5B
NEU1
NEU2
NEU3
NEU4
OGT
POFUT1
POFUT2
POGLUT1
POGLUT2
POGLUT3
POMGNT1
POMGNT2
POMT1
POMT2
RFNG
RXYLT1
ST3GAL1
ST3GAL2
ST3GAL3
ST3GAL4
ST3GAL6
ST6GAL1
ST6GAL2
ST6GALNAC1
ST6GALNAC2
ST6GALNAC3
ST6GALNAC4
ST8SIA2
ST8SIA3
ST8SIA4
ST8SIA6
TMTC1
TMTC2
TMTC3
TMTC4
XXYLT1
Pathway Map
B3GALNT2



General Information
Gene Symbol:   
B3GALNT2
Protein name:   
UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.313
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• ER resident enzyme.
• Adds GalNAc to create Type I LacdiNAc structures on both N-glycans and O-glycans, but not glycolipid substrates.
• In particular it forms O-Mannosylated structures on α-dystroglycans, and this is necessary for laminin binding. Thus, it is a critical enzyme that is required for normal development of muscle and brain.
• Other enzyme substrates are yet unidentified.

Related Pathway
KEGG ORTHOLOGY:   
K09654
KEGG Pathway:   
ko00515  ko01100 
Reactome:   
R-HSA-5173105
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8NCR0 
NCBI GeneID:   
148789
GlyMap GeneID:   
148789
DNA aRefSeq:   
NM_001277155.2 
Protein RefSeq:   
NP_001264084.1 
BRENDA:   
Kinetic info.
OMIM number:   
610194
GeneCards database:   
B3GALNT2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-?) or GlcNAc(b1-4)...Man(a1-?)Ser|Thr
Product:  
     GalNAc(b1-3)GlcNAc(b1-?) or GalNAc(b1-3)GlcNAc(b1-4)...Man(a1-?)Ser|Thr
Constraints:  
     !@GlcNAc(b1-4)...Man(b1-4)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    5.75e-04    (pmol/cell • hr)       KM:    1.50e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4467 2660-2667 -0.551 98.0 -0.366 95.0 noncon
hsa-miR-548v 190-197 -0.505 99.0 -0.505 99.0 noncon
hsa-miR-1282 38-45 -0.486 97.0 -0.486 98.0 noncon
hsa-miR-4763-5p 33-40 -0.48 97.0 -0.48 98.0 noncon
hsa-miR-7976 29-36 -0.452 99.0 -0.452 99.0 noncon
hsa-miR-645 1277-1284 -0.425 97.0 -0.294 95.0 noncon
hsa-miR-19a-3p 158-165 -0.391 97.0 -0.391 98.0 con
hsa-miR-19b-3p 158-165 -0.391 97.0 -0.391 98.0 con
hsa-miR-4711-5p 280-287 -0.382 98.0 -0.361 98.0 noncon
hsa-miR-6894-3p 34-41 -0.382 98.0 -0.382 99.0 noncon
hsa-miR-505-3p.1 789-796 -0.373 96.0 -0.34 96.0 noncon
hsa-miR-218-1-3p 575-582 -0.373 97.0 -0.353 97.0 noncon
hsa-miR-4477b 170-177 -0.369 98.0 -0.369 98.0 noncon
hsa-miR-449b-3p 289-296 -0.369 98.0 -0.349 99.0 noncon
hsa-miR-3192-3p 1225-1232 -0.36 99.0 -0.253 97.0 noncon
hsa-miR-187-3p 1400-1407 -0.354 90.0 -0.247 86.0 noncon
hsa-miR-4753-5p 2596-2603 -0.352 97.0 -0.24 95.0 noncon
hsa-miR-6770-3p 2661-2667 -0.347 91.0 -0.236 85.0 noncon
hsa-miR-6760-3p 2400-2407 -0.336 98.0 -0.229 96.0 noncon
hsa-miR-503-3p 638-645 -0.331 96.0 -0.302 97.0 noncon
hsa-miR-101-3p.1 187-194 -0.331 97.0 -0.331 98.0 noncon
hsa-miR-6768-3p 2996-3002 -0.33 98.0 -0.112 89.0 noncon
hsa-miR-7703 52-59 -0.325 98.0 -0.325 98.0 noncon
hsa-miR-4425 1295-1302 -0.325 94.0 -0.227 89.0 noncon
hsa-miR-6776-5p 128-135 -0.324 97.0 -0.324 98.0 noncon
hsa-miR-4762-3p 231-238 -0.311 98.0 -0.311 99.0 noncon
hsa-miR-125a-5p 748-754 -0.311 96.0 -0.284 96.0 con
hsa-miR-7702 20-27 -0.304 95.0 -0.304 97.0 noncon
hsa-miR-6732-5p 1341-1347 -0.301 93.0 -0.211 89.0 noncon
hsa-miR-3150a-3p 1008-1015 -0.301 96.0 -0.275 96.0 noncon
hsa-miR-125b-2-3p 2956-2963 -0.3 98.0 -0.205 96.0 noncon
hsa-miR-7114-5p 1475-1482 -0.299 97.0 -0.209 95.0 noncon
hsa-miR-2053 3043-3050 -0.296 99.0 0.0 0.0 noncon
hsa-miR-4753-5p 2995-3001 -0.294 96.0 -0.101 81.0 noncon
hsa-miR-125b-5p 748-754 -0.289 95.0 -0.264 95.0 con
hsa-miR-4522 17-23 -0.287 95.0 -0.287 97.0 noncon
hsa-miR-342-3p 720-726 -0.283 97.0 -0.259 97.0 noncon
hsa-miR-4319 748-754 -0.267 94.0 -0.244 94.0 con
hsa-miR-6728-3p 15-21 -0.267 96.0 -0.267 97.0 noncon
hsa-miR-199a-5p 2552-2559 -0.265 93.0 -0.182 91.0 noncon
hsa-miR-199b-5p 2552-2559 -0.265 93.0 -0.182 91.0 noncon
hsa-miR-141-5p 223-229 -0.262 95.0 -0.262 96.0 noncon
hsa-miR-4715-5p 2628-2635 -0.262 92.0 -0.18 90.0 noncon
hsa-miR-3144-3p 904-911 -0.262 93.0 -0.24 95.0 noncon
hsa-miR-6763-5p 1008-1015 -0.261 93.0 -0.239 93.0 noncon
hsa-miR-433-3p 1181-1188 -0.254 96.0 -0.233 97.0 noncon
hsa-miR-6719-3p 524-531 -0.253 96.0 -0.24 97.0 noncon
hsa-miR-4266 821-827 -0.253 90.0 -0.232 92.0 noncon
hsa-miR-1261 2949-2956 -0.252 94.0 -0.173 90.0 noncon