GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ARSB



General Information
Gene Symbol:   
ARSB
Protein name:   
Arylsulfatase B
Enzyme Class:   
CAZy Family:   
E.C. No:   
3.1.6.12
Organs:   
Reaction (SNFG):   

GalNAc(b1-4 [4S]) → GalNAc(b1-4)
Reaction (IUBMB):   
No specified reaction.
About

• Removes sulfate groups from chondroitin-4-sulfate(C4S) and regulates its degradation
• One of three human CS/DS exosulfatases: ARSB (4-O-sulfatase) (Bond et al., 1997), GALNS (6-O-sulfatase) (Rivera-Colon et al., 2012) and IDS (iduronate-2-O-sulfatase) (Demydchuk et al., 2017).

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
P15848 
NCBI GeneID:   
411
GlyMap GeneID:   
411
DNA aRefSeq:   
NM_000046.3 
Protein RefSeq:   
NP_000037.2 
BRENDA:   
Kinetic info.
OMIM number:   
611542
GeneCards database:   
ARSB
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     
Substrate:  
     GalNAc(b1-4 [4S])
Product:  
     GalNAc(b1-4)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
No expression data available.
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
ARSB/hsa-miR-5693
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...UAAAUGCAAUUACU GCCACUGA ...
3'   CUCAAGUAAAGUCU CGGUGAC G
 8mer 3035-3042 -0.263 98.0 -0.056 87.0 noncon
5'...GAUUACAGGUGUGA GCCACUG U...
3'   CUCAAGUAAAGUCU CGGUGAC G
 7mer-m8 2811-2817 -0.028 72.0 -0.006 59.0 noncon