FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
AGA



General Information
Gene Symbol:   
AGA
Protein name:   
N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase
Enzyme Class:   
CAZy Family:   
E.C. No:   
3.5.1.26
Organs:   
Reaction (SNFG):   

...<[...]>GlcNAc(b1-?)Asn → ...<[...]>GlcNAc(b1-?)
Reaction (IUBMB):   
No specified reaction.
About

• Amidohydrolase involved in lysosomal catabolism of N-linked glycans.
• It releases the glycan from proteolized/unfolded protein.
• Enzyme deficiency causes the lysosomal storage disease aspartylglycosaminuria.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-6798695
Related Database
Organism:   
Homo sapiens
Uniprot:   
P20933 
NCBI GeneID:   
175
GlyMap GeneID:   
175
DNA aRefSeq:   
NM_000027.3 
Protein RefSeq:   
NP_000018.2 
BRENDA:   
Kinetic info.
OMIM number:   
613228
GeneCards database:   
AGA
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     ...<[...]>GlcNAc(b1-?)Asn
Product:  
     ...<[...]>GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-487b-3p 921-928 -0.858 99.0 -0.08 58.0 noncon
hsa-miR-4524a-5p 661-668 -0.592 99.0 -0.059 71.0 noncon
hsa-miR-4524b-5p 661-668 -0.576 99.0 -0.058 70.0 noncon
hsa-miR-5683 18-25 -0.563 99.0 -0.563 99.0 noncon
hsa-miR-4693-3p 63-70 -0.539 99.0 -0.539 99.0 noncon
hsa-miR-5010-3p 116-123 -0.526 99.0 -0.526 99.0 noncon
hsa-miR-646 660-667 -0.51 99.0 -0.052 73.0 noncon
hsa-miR-3928-3p 80-87 -0.509 99.0 -0.509 99.0 noncon
hsa-miR-6839-3p 321-328 -0.484 98.0 -0.061 74.0 noncon
hsa-miR-383-5p.1 264-271 -0.479 99.0 -0.06 60.0 noncon
hsa-miR-4474-3p 636-643 -0.445 98.0 -0.047 64.0 noncon
hsa-miR-629-5p 320-327 -0.435 99.0 -0.056 73.0 noncon
hsa-miR-6505-3p 765-772 -0.423 98.0 -0.045 64.0 noncon
hsa-miR-1252-5p 873-880 -0.419 99.0 -0.044 64.0 noncon
hsa-miR-4659a-3p 31-38 -0.414 99.0 -0.414 99.0 noncon
hsa-miR-4659b-3p 31-38 -0.414 99.0 -0.414 99.0 noncon
hsa-miR-3939 848-854 -0.411 92.0 -0.043 27.0 noncon
hsa-miR-7112-3p 443-450 -0.404 98.0 -0.052 72.0 noncon
hsa-miR-145-3p 330-337 -0.401 99.0 -0.052 75.0 noncon
hsa-miR-3667-3p 499-506 -0.391 98.0 -0.051 73.0 noncon
hsa-miR-101-3p.1 718-725 -0.378 98.0 -0.04 77.0 noncon
hsa-miR-192-3p 139-145 -0.343 98.0 -0.343 98.0 noncon
hsa-miR-3942-3p 154-161 -0.342 99.0 -0.045 82.0 noncon
hsa-miR-597-5p 250-256 -0.342 97.0 -0.045 68.0 noncon
hsa-miR-5590-5p 755-762 -0.33 98.0 -0.036 58.0 noncon
hsa-miR-1267 896-902 -0.329 98.0 -0.036 62.0 noncon
hsa-miR-4704-5p 75-81 -0.327 99.0 -0.327 99.0 noncon
hsa-miR-943 899-905 -0.327 97.0 -0.036 67.0 noncon
hsa-miR-4691-3p 218-224 -0.321 97.0 -0.042 77.0 noncon
hsa-miR-4272 134-140 -0.318 99.0 -0.318 99.0 noncon
hsa-miR-7161-5p 98-104 -0.313 99.0 -0.313 99.0 noncon
hsa-miR-562 287-293 -0.313 98.0 -0.041 77.0 noncon
hsa-miR-4999-5p 552-558 -0.31 95.0 -0.041 58.0 noncon
hsa-miR-4503 349-356 -0.309 96.0 -0.041 62.0 noncon
hsa-miR-6730-5p 184-190 -0.301 98.0 -0.04 73.0 noncon
hsa-miR-5584-5p 799-806 -0.296 98.0 -0.032 65.0 noncon
hsa-miR-561-3p 932-939 -0.293 99.0 -0.012 67.0 noncon
hsa-miR-1272 335-342 -0.291 98.0 -0.039 76.0 noncon
hsa-miR-3915 81-87 -0.291 97.0 -0.291 98.0 noncon
hsa-miR-548u 98-104 -0.288 98.0 -0.288 99.0 noncon
hsa-miR-4318 306-312 -0.283 97.0 -0.038 70.0 noncon
hsa-miR-7114-5p 305-311 -0.273 97.0 -0.037 62.0 noncon
hsa-miR-5687 91-97 -0.273 98.0 -0.273 99.0 noncon
hsa-miR-3157-5p 47-53 -0.268 96.0 -0.268 97.0 noncon
hsa-miR-4522 397-403 -0.262 94.0 -0.035 59.0 noncon
hsa-miR-503-5p 661-667 -0.26 97.0 -0.029 58.0 noncon
hsa-miR-377-3p 620-627 -0.259 94.0 -0.029 55.0 noncon
hsa-miR-4740-5p 413-419 -0.257 93.0 -0.035 47.0 noncon
hsa-miR-449b-3p 217-223 -0.257 96.0 -0.035 67.0 noncon
hsa-miR-216a-5p 294-300 -0.255 97.0 -0.034 68.0 noncon
hsa-miR-4329 401-407 -0.253 97.0 -0.034 79.0 noncon
hsa-miR-5684 358-364 -0.252 96.0 -0.034 63.0 noncon
hsa-miR-28-3p 73-79 -0.251 96.0 -0.251 97.0 noncon
hsa-miR-548ap-3p 469-476 -0.25 98.0 -0.034 79.0 noncon
hsa-miR-548aa 469-476 -0.25 98.0 -0.034 79.0 noncon
hsa-miR-548t-3p 469-476 -0.25 98.0 -0.034 79.0 noncon