GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
A4GNT



General Information
Gene Symbol:   
A4GNT
Protein name:   
Alpha-1,4-N-acetylglucosaminyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT32
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Transfers GlcNAc(α1-4) to βGal preferentially present in O-glycans forming GlcNAc(α1-4)Galβ-R or terminal αGlcNAc.
• The enzyme shows preference to act on core-2 glycan rather than core-1 glycans indicating a role for GlcNAc(β1-6) in promoting its activity. It extends the core-1 arm of O-glycans.
• It can act on both arms of core-2 glycan to corm structures like GlcNAc(α1-4)Gal(β1-4)GlcNAc(β1-6)[GlcNAc(α1-4)Gal(β1-3)]GalNAc.
• A4GNT is expressed in stomach, gall bladder, duodenum, and thus such structures are normally restricted to gland & gastric mucins, particulalry MUC6 with a small amount also being associated with MUC5AC.
• αGlcNAc is also expressed in pancreatic ducts showing gastric metaplasia or pancreatic intraepithelial neoplasia 1 (PanIN-1).
• A4gnt knockout mice revealed that αGlcNAc serves as a tumor suppressor of differentiated type of gastric adenocarcinoma. In addition, αGlcNAc protects gastric gland mucous cells from H. pylori infection. Thus, αGlcNAc plays a dual role to prevent the gastric mucosa from gastric tumorigenesis.

Related Pathway
KEGG ORTHOLOGY:   
K08251
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-913709
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9UNA3 
NCBI GeneID:   
51146
GlyMap GeneID:   
51146
DNA aRefSeq:   
NM_016161.2 
Protein RefSeq:   
NP_057245.1 
BRENDA:   
Kinetic info.
OMIM number:   
-
GeneCards database:   
A4GNT
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type)
Compartment:  
     
Substrate:  
     Gal(b1-?)
Product:  
     GlcNAc(a1-4)Gal(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 12 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000236709 ZFX PPARG NFIC CTCF MYC ZNF143 DNMT3B
miRNA-GlycoEnzyme Interactome {ref. 9}
A4GNT/hsa-miR-4455
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AUAAAACAU ACACCCA U...
3'   UUUUUGUGUG UGUGGG A
 7mer-A1 183-189 -0.332 91.0 -0.332 94.0 noncon